Journal: bioRxiv

Article Title: The mechanism of Micafungin action to Pteropine orthoreovirus infection in human cell line

doi: 10.1101/2025.01.23.634615

Figure Lengend Snippet: IL-6 gene expression analysis with siRNA against IL-6 and MCFG. Values were calculated using the 2 -ΔΔCt comparative method. Statistical differences between groups by a student’s t-test. Bars represent the mean (n=3) relative gene expression from three independent experiments with standard deviation (*; p -value < 0.05, **; p < 0.01, ***; p < 0.001, ****; p < 0.0001) (A). Viral copy numbers by qPCR, with data represented as the mean (n=3) (B). Virus titer in the supernatant by TCID□□ assay (n=3) (C). The percentage of syncytial formation area was calculated using ImageJ software (n=12) (D). TNFSF13B gene expression analysis with siRNA against IL-6 and MCFG (E), and ICAM1 gene (F).

Article Snippet: The sequences of the siRNAs are as follows: siRNA IL-6 sense: 5’-GGACAUGACAACUCAUCUCtt-3’, siRNA IL-6 antisense: 5’-GAGAUGAGUUGUCAUGUCCtt-3’, siRNA scrambled control sense: 5’-AUUGGGUAGUGUUUCAGGCtt-3’, and siRNA scrambled control antisense: 5’-ACGUGACACGUUCGGAGAAtt-3’ (FASMAC, Kanagawa, Japan).

Techniques: Gene Expression, Standard Deviation, Virus, Software

Journal: bioRxiv

Article Title: A QbD strategy to develop curcumin and siRNA co-loaded lipoplexes to combat osteoarthritis-related inflammation and oxidative stress

doi: 10.1101/2024.11.25.625050

Figure Lengend Snippet:

Article Snippet: For Lipoplexes, IL-6 siRNA (Cat.No: 4390824 - s7313), IL-8 siRNA (Cat.No: 4390824 - s7328), Scramble (negative control) siRNA (Cat.No: 4390843) were obtained from ThermoFisher Scientific (Eugene, USA).

Techniques:

Journal: bioRxiv

Article Title: A QbD strategy to develop curcumin and siRNA co-loaded lipoplexes to combat osteoarthritis-related inflammation and oxidative stress

doi: 10.1101/2024.11.25.625050

Figure Lengend Snippet: (a) IL-6 mRNA levels in the C28/I2 cell line were expressed through qRT-PCR, and (b) protein levels were quantified using ELISA. (c) IL-8 mRNA levels in the C28/I2 cell line were expressed through qRT-PCR, and (d) protein levels were quantified using ELISA. Positive control (PC), negative control (NC), curcumin-loaded cationic liposomes (Opt-CL), IL-6 and IL-8 siRNA + Curcumin co-loaded lipoplexes (ILsiRNA-CLx), Scramble siRNA + Curcumin co-loaded lipoplexes (SsiRNA-CLx), IL-6 and IL-8 siRNA lipoplexes (ILsiRNA-Lx), Scramble siRNA lipoplexes (SsiRNA-Lx), and IL-6 and IL-8 siRNA with Lipofectamine RNAimax formulation as a standard for transfection and knockdown (ILsiRNA-LR). Results were expressed as mean ± SD of three independent measurements. A one-way ANOVA test with multiple comparisons was performed to analyze the differences between the effects of the treatments (ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). The graphs were generated using GraphPad Prism 10.2.2.

Article Snippet: For Lipoplexes, IL-6 siRNA (Cat.No: 4390824 - s7313), IL-8 siRNA (Cat.No: 4390824 - s7328), Scramble (negative control) siRNA (Cat.No: 4390843) were obtained from ThermoFisher Scientific (Eugene, USA).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control, Liposomes, Formulation, Transfection, Knockdown, Generated

Journal: bioRxiv

Article Title: A QbD strategy to develop curcumin and siRNA co-loaded lipoplexes to combat osteoarthritis-related inflammation and oxidative stress

doi: 10.1101/2024.11.25.625050

Figure Lengend Snippet: IL-6 mRNA levels were expressed through qRT-PCR (left), and protein levels were quantified using ELISA (right). a&b – primary chondrocytes C28-CA-22-004, c&d – primary chondrocytes C28-CA-22-009, e&f – primary chondrocytes C28-CA-23-004. Positive control (PC), negative control (NC), curcumin-loaded cationic liposomes (Opt-CL), IL-6 and IL-8 siRNA + Curcumin co-loaded lipoplexes (ILsiRNA-CLx), Scramble siRNA + Curcumin co-loaded lipoplexes (SsiRNA-CLx), IL-6 and IL-8 siRNA lipoplexes (ILsiRNA-Lx), Scramble siRNA lipoplexes (SsiRNA-Lx), and IL-6 and IL-8 siRNA with Lipofectamine RNAimax formulation as a standard for transfection and knockdown (ILsiRNA-LR). Results were expressed as mean ± SD of three independent measurements. A one-way ANOVA test with multiple comparisons was performed to analyze the differences between the effects of the treatments (ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). The graphs were generated using GraphPad Prism 10.2.2.

Article Snippet: For Lipoplexes, IL-6 siRNA (Cat.No: 4390824 - s7313), IL-8 siRNA (Cat.No: 4390824 - s7328), Scramble (negative control) siRNA (Cat.No: 4390843) were obtained from ThermoFisher Scientific (Eugene, USA).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control, Liposomes, Formulation, Transfection, Knockdown, Generated

Journal: bioRxiv

Article Title: A QbD strategy to develop curcumin and siRNA co-loaded lipoplexes to combat osteoarthritis-related inflammation and oxidative stress

doi: 10.1101/2024.11.25.625050

Figure Lengend Snippet: IL-8 mRNA levels were expressed through qRT-PCR (left), and protein levels were quantified using ELISA (right). a&b – primary chondrocytes C28-CA-22-004, c&d – primary chondrocytes C28-CA-22-009, e&f – primary chondrocytes C28-CA-23-004. Positive control (PC), negative control (NC), curcumin-loaded cationic liposomes (Opt-CL), IL-6 and IL-8 siRNA + Curcumin co-loaded lipoplexes (ILsiRNA-CLx), Scramble siRNA + Curcumin co-loaded lipoplexes (SsiRNA-CLx), IL-6 and IL-8 siRNA lipoplexes (ILsiRNA-Lx), Scramble siRNA lipoplexes (SsiRNA-Lx), and IL-6 and IL-8 siRNA with Lipofectamine RNAimax formulation as a standard for transfection and knockdown (ILsiRNA-LR). Results were expressed as mean ± SD of three independent measurements. A one-way ANOVA test with multiple comparisons was performed to analyze the differences between the effects of the treatments (ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001). The graphs were generated using GraphPad Prism 10.2.2.

Article Snippet: For Lipoplexes, IL-6 siRNA (Cat.No: 4390824 - s7313), IL-8 siRNA (Cat.No: 4390824 - s7328), Scramble (negative control) siRNA (Cat.No: 4390843) were obtained from ThermoFisher Scientific (Eugene, USA).

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Positive Control, Negative Control, Liposomes, Formulation, Transfection, Knockdown, Generated